Dynamics of Escherichia coli type I-E CRISPR spacers over 42 000 years
نویسندگان
چکیده
منابع مشابه
Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system
BACKGROUND Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is a recently developed powerful tool for gene regulation. In Escherichia coli, the type I CRISPR system expressed endogenously shall be easy for internal regulation without causing metabolic burden in compared with the widely used type II system, which expressed dCas9 as an additional plasmid. RESULTS...
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ژورنال
عنوان ژورنال: Molecular Ecology
سال: 2017
ISSN: 0962-1083
DOI: 10.1111/mec.13961